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#The importance of serial dilution download

If testing a pregnant female, each month serum should be compared to the previous month.

If titrating Rh, Kell, Duffy, or Kidd antibodies, the serologic technique includes a 37 oC followed by antiglobulin testing.

If titrating anti-A, anti-B, or anti-A,B, the serologic technique is performed by the same method as ABO Typing.

Examine test results macroscopically, grade and record the reactions NOTES AND PRECAUTIONS.

Mix well and test by the appropriate technique for the specific antibody.

Add one drops of specific red blood cells.

Using a separate pipettes for each dilution, transfer 2 drops of each tube to the appropriate tubes.

Label a new set of 10 tubes with the appropriate dilutions.

Remove 0.3 ml from tube 10 (512) and reserve in a clean tube if the titration needs to be continued.

Continue the process for all dilutions (512).

Use a clean pipette to mix the 1/4 dilution several times and then transfer 0.3 ml to tube 8.

Use a clean pipette to mix the 1/2 dilution several times and then transfer 0.3 ml to tube 4.

Add 0.3 ml of serum to both tubes 1 and 2.

Add 0.3 ml of saline to tubes 2 through 512.Tube 2 will be a 1/2 dilution, 4 will be a 1/4 dilution. Importance Of Serial Dilution In Serology Definition. What is the importance of serial dilution in serological test? Serial Dilution: Importance and Aplication. Label 10 tubes according to the serial dilution: 1, 2, 4, 8, 16, 32, 64, 128, 256, 512 and the patient identification.lighted agglutination viewer PROCEDURE.If titering for anti-D levels, O cells that are homozygous for D should be used. If titering for anti-A or anti-B levels, A 1 or B Cells would be used. Reagent cells known to have the antigen that will be reacting with the titered antibody.In the case of a pregnant female, frozen serum or plasma from previous months should be run along with the fresh specimen to determine if there is a rise in titer. characterizing antibodies that may be high titer, low avidity antibodies SAMPLE Either plasma and serum can be titrated.

attempting to determine if there is any specificity to an autoantibody.estimating the antibody activity of an alloimmunized pregnant female.The usual applications of titration studies are:

#The importance of serial dilution download

Through The Fire Sebastian Telfair Download Free. If the concentration of the specific antibody is being determined, the cells must contain the known antigen and the procedure should be performed under the optimal conditions for that antibody. › ▀ Importance Of Serial Dilution In Serology TestsĪBO Antibody Titration Antibody Titration PRINCIPLE AND APPLICATIONS: Antibody titration is a determination of the concentration of a specific antibody in the patient's serum or to determine the strength of antigen expression on different red cell samples.